Lab Publications

Gastric inhibitory peptide controls adipose insulin sensitivity via activation of cAMP-response element-binding protein and p110β isoform of phosphatidylinositol 3-kinase. Mohammad S, Ramos LS, Buck J, Levin LR, Rubino F, McGraw TE. J Biol Chem. 2011 Dec 16;286(50):43062-70.

Hyperinsulinemia leads to uncoupled insulin regulation of the GLUT4 glucose transporter and the FoxO1 transcription factor. Gonzalez E, Flier E, Molle D, Accili D, McGraw TE. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10162-7.

Rosiglitazone, PPARγ, and type 2 diabetes. Kahn BB, McGraw TE. N Engl J Med. 2010 Dec 30;363(27):2667-9.

Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Jordens I, Molle D, Xiong W, Keller SR, McGraw TE. Mol Biol Cell. 2010 Jun 15;21(12):2034-44.

GLUT4 is sorted to vesicles whose accumulation beneath and insertion into the plasma membrane are differentially regulated by insulin and selectively affected by insulin resistance.

Xiong W, Jordens I, Gonzalez E, McGraw TE.

Mol Biol Cell. 2010 Apr 15;21(8):1375-86.

Stem cells: Metabolism regulates differentiation.

McGraw TE, Mittal V.

Nat Chem Biol. 2010 Mar;6(3):176-177.

Metabolic surgery: the role of the gastrointestinal tract in diabetes mellitus.

Rubino F, R'bibo SL, del Genio F, Mazumdar M, McGraw TE.

Nat Rev Endocrinol. 2010 Feb;6(2):102-9.

The Akt kinases: isoform specificity in metabolism and cancer.

Gonzalez E, McGraw TE.

Cell Cycle. 2009 Aug 15;8(16):2502-8. Epub 2009 Aug 23

Insulin-modulated Akt subcellular localization determines Akt isoform-specific signaling.

Gonzalez E, McGraw TE.

Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7004-9.

Use of quantitative immunofluorescence microscopy to study intracellular trafficking: studies of the GLUT4 glucose transporter.

Blot V, McGraw TE.

Methods Mol Biol. 2008;457:347-66.

The CB1 endocannabinoid system modulates adipocyte insulin sensitivity.

Motaghedi R, McGraw TE.

Obesity (Silver Spring). 2008 Aug;16(8):1727-34.

Molecular mechanisms controlling GLUT4 intracellular retention.

Blot V, McGraw TE.

Mol Biol Cell. 2008 Aug;19(8):3477-87.

Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism.

Zeigerer A, Rodeheffer MS, McGraw TE, Friedman JM.

Exp Cell Res. 2008 Jul 1;314(11-12):2249-56.

Endocytosis: biochemical analyses.

McGraw TE, Subtil A.

Curr Protoc Cell Biol. 2001 May

Rab10, a target of the AS160 Rab GAP, is required for insulin-stimulated translocation of GLUT4 to the adipocyte plasma membrane.

Sano H, Eguez L, Teruel MN, Fukuda M, Chuang TD, Chavez JA, Lienhard GE, McGraw TE.

Cell Metab. 2007 Apr;5(4):293-303.

GLUT4 is internalized by a cholesterol-dependent nystatin-sensitive mechanism inhibited by insulin.

Blot V, McGraw TE.

EMBO J. 2006 Dec 13;25(24):5648-58.

Insulin signaling diverges into Akt-dependent and -independent signals to regulate the recruitment/docking and the fusion of GLUT4 vesicles to the plasma membrane.

Gonzalez E, McGraw TE.

Mol Biol Cell. 2006 Oct;17(10):4484-93.

GLUT4 distribution between the plasma membrane and the intracellular compartments is maintained by an insulin-modulated bipartite dynamic mechanism.

Martin OJ, Lee A, McGraw TE.

J Biol Chem. 2006 Jan 6;281(1):484-90.

Full intracellular retention of GLUT4 requires AS160 Rab GTPase activating protein.

Eguez L, Lee A, Chavez JA, Miinea CP, Kane S, Lienhard GE, McGraw TE.

Cell Metab. 2005 Oct;2(4):263-72.

The transcription factor CCAAT/enhancer-binding protein alpha is required for the intracellular retention of GLUT4.

Wertheim N, Cai Z, McGraw TE.

J Biol Chem. 2004 Oct 1;279(40):41468-76.

Insulin stimulation of GLUT4 exocytosis, but not its inhibition of endocytosis, is dependent on RabGAP AS160.

Zeigerer A, McBrayer MK, McGraw TE.

Mol Biol Cell. 2004 Oct;15(10):4406-15.

Chemical genetic screening identifies sulfonamides that raise organellar pH and interfere with membrane traffic.

Nieland TJ, Feng Y, Brown JX, Chuang TD, Buckett PD, Wang J, Xie XS, McGraw TE, Kirchhausen T, Wessling-Resnick M.

Traffic. 2004 Jul;5(7):478-92.

Endocytic recycling.

Maxfield FR, McGraw TE.

Nat Rev Mol Cell Biol. 2004 Feb;5(2):121-32.

Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.

Kuliawat R, Kalinina E, Bock J, Fricker L, McGraw TE, Kim SR, Zhong J, Scheller R, Arvan P.

Mol Biol Cell. 2004 Apr;15(4):1690-701.

GLUT4 is retained by an intracellular cycle of vesicle formation and fusion with endosomes.

Karylowski O, Zeigerer A, Cohen A, McGraw TE.

Mol Biol Cell. 2004 Feb;15(2):870-82.

Targeted chemical disruption of clathrin function in living cells.

Moskowitz HS, Heuser J, McGraw TE, Ryan TA.

Mol Biol Cell. 2003 Nov;14(11):4437-47

Evidence for the interaction of the hereditary haemochromatosis protein, HFE, with the transferrin receptor in endocytic compartments.

Davies PS, Zhang AS, Anderson EL, Roy CN, Lampson MA, McGraw TE, Enns CA.

Biochem J. 2003 Jul 1;373(Pt 1):145-53.

CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl.

Cormont M, Metón I, Mari M, Monzo P, Keslair F, Gaskin C, McGraw TE, Le Marchand-Brustel Y.

Traffic. 2003 Feb;4(2):97-112.

GLUT4 retention in adipocytes requires two intracellular insulin-regulated transport steps.

Zeigerer A, Lampson MA, Karylowski O, Sabatini DD, Adesnik M, Ren M, McGraw TE.

Mol Biol Cell. 2002 Jul;13(7):2421-35.

The epithelial-specific adaptor AP1B mediates post-endocytic recycling to the basolateral membrane.

Gan Y, McGraw TE, Rodriguez-Boulan E.

Nat Cell Biol. 2002 Aug;4(8):605-9.

Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles.

Lampson MA, Schmoranzer J, Zeigerer A, Simon SM, McGraw TE.

Mol Biol Cell. 2001 Nov;12(11):3489-501.

Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle.

Hao M, Lin SX, Karylowski OJ, Wüstner D, McGraw TE, Maxfield FR.

J Biol Chem. 2002 Jan 4;277(1):609-17.

A di-leucine sequence and a cluster of acidic amino acids are required for dynamic retention in the endosomal recycling compartment of fibroblasts.

Johnson AO, Lampson MA, McGraw TE.

Mol Biol Cell. 2001 Feb;12(2):367-81.

Demonstration of insulin-responsive trafficking of GLUT4 and vpTR in fibroblasts.

Lampson MA, Racz A, Cushman SW, McGraw TE.

J Cell Sci. 2000 Nov;113 ( Pt 22):4065-76.

Characterization of the insulin-regulated endocytic recycling mechanism in 3T3-L1 adipocytes using a novel reporter molecule.

Subtil A, Lampson MA, Keller SR, McGraw TE.

J Biol Chem. 2000 Feb 18;275(7):4787-95.

Acute cholesterol depletion inhibits clathrin-coated pit budding.

Subtil A, Gaidarov I, Kobylarz K, Lampson MA, Keen JH, McGraw TE.

Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6775-80.

An endocytosed TGN38 chimeric protein is delivered to the TGN after trafficking through the endocytic recycling compartment in CHO cells.

Ghosh RN, Mallet WG, Soe TT, McGraw TE, Maxfield FR.

J Cell Biol. 1998 Aug 24;142(4):923-36.

Identification of an insulin-responsive, slow endocytic recycling mechanism in Chinese hamster ovary cells.

Johnson AO, Subtil A, Petrush R, Kobylarz K, Keller SR, McGraw TE.

J Biol Chem. 1998 Jul 10;273(28):17968-77.

The transferrin receptor cytoplasmic domain determines its rate of transport through the biosynthetic pathway and its susceptibility to cleavage early in the pathway.

Rutledge EA, Gaston I, Root BJ, McGraw TE, Enns CA.

J Biol Chem. 1998 May 15;273(20):12169-75.

Overexpression of MAP4 inhibits organelle motility and trafficking in vivo.

Bulinski JC, McGraw TE, Gruber D, Nguyen HL, Sheetz MP.

J Cell Sci. 1997 Dec;110 ( Pt 24):3055-64.

Bafilomycin A1 treatment retards transferrin receptor recycling more than bulk membrane recycling.

Presley JF, Mayor S, McGraw TE, Dunn KW, Maxfield FR.

J Biol Chem. 1997 May 23;272(21):13929-36.

Transferrin receptor containing the SDYQRL motif of TGN38 causes a reorganization of the recycling compartment but is not targeted to the TGN.

Johnson AO, Ghosh RN, Dunn KW, Garippa R, Park J, Mayor S, Maxfield FR, McGraw TE.

J Cell Biol. 1996 Dec;135(6 Pt 2):1749-62.

The carboxyl terminus of GLUT4 contains a serine-leucine-leucine sequence that functions as a potent internalization motif in Chinese hamster ovary cells.

Garippa RJ, Johnson A, Park J, Petrush RL, McGraw TE.

J Biol Chem. 1996 Aug 23;271(34):20660-8.

Wortmannin-sensitive trafficking pathways in Chinese hamster ovary cells. Differential effects on endocytosis and lysosomal sorting.

Martys JL, Wjasow C, Gangi DM, Kielian MC, McGraw TE, Backer JM.

J Biol Chem. 1996 May 3;271(18):10953-62.

Studies of transferrin recycling reconstituted in streptolysin O permeabilized Chinese hamster ovary cells.

Martys JL, Shevell T, McGraw TE.

J Biol Chem. 1995 Oct 27;270(43):25976-84.

An internalization motif is created in the cytoplasmic domain of the transferrin receptor by substitution of a tyrosine at the first position of a predicted tight turn.

Pytowski B, Judge TW, McGraw TE.

J Biol Chem. 1995 Apr 21;270(16):9067-73.

The amino terminus of GLUT4 functions as an internalization motif but not an intracellular retention signal when substituted for the transferrin receptor cytoplasmic domain.

Garippa RJ, Judge TW, James DE, McGraw TE.

J Cell Biol. 1994 Mar;124(5):705-15.

Regulation of endocytic trafficking and acidification are independent of the cystic fibrosis transmembrane regulator.

Dunn KW, Park J, Semrad CE, Gelman DL, Shevell T, McGraw TE.

J Biol Chem. 1994 Feb 18;269(7):5336-45.

Slowed receptor trafficking in mutant CHO lines of the End1 and End2 complementation groups.

Johnson LS, Presley JF, Park JC, McGraw TE.

J Cell Physiol. 1994 Jan;158(1):29-38.

Endosome acidification and receptor trafficking: bafilomycin A1 slows receptor externalization by a mechanism involving the receptor's internalization motif.

Johnson LS, Dunn KW, Pytowski B, McGraw TE.

Mol Biol Cell. 1993 Dec;4(12):1251-66.

The End2 mutation in CHO cells slows the exit of transferrin receptors from the recycling compartment but bulk membrane recycling is unaffected.

Presley JF, Mayor S, Dunn KW, Johnson LS, McGraw TE, Maxfield FR.

J Cell Biol. 1993 Sep;122(6):1231-41.

Isolation of a temperature-sensitive variant Chinese hamster ovary cell line with a morphologically altered endocytic recycling compartment.

McGraw TE, Dunn KW, Maxfield FR.

J Cell Physiol. 1993 Jun;155(3):579-94.

Mutagenesis of the human transferrin receptor: two cytoplasmic phenylalanines are required for efficient internalization and a second-site mutation is capable of reverting an internalization-defective phenotype.

McGraw TE, Pytowski B, Arzt J, Ferrone C.

J Cell Biol. 1991 Mar;112(5):853-61.

Human transferrin receptor internalization is partially dependent upon an aromatic amino acid on the cytoplasmic domain.

McGraw TE, Maxfield FR.

Cell Regul. 1990 Mar;1(4):369-77.

Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome.

Dunn KW, McGraw TE, Maxfield FR.

J Cell Biol. 1989 Dec;109(6 Pt 2):3303-14.

Phorbol ester treatment increases the exocytic rate of the transferrin receptor recycling pathway independent of serine-24 phosphorylation.

McGraw TE, Dunn KW, Maxfield FR.

J Cell Biol. 1988 Apr;106(4):1061-6.

Functional expression of the human transferrin receptor cDNA in Chinese hamster ovary cells deficient in endogenous transferrin receptor.

McGraw TE, Greenfield L, Maxfield FR.

J Cell Biol. 1987 Jul;105(1):207-14.